![](https://parts.igem.org/images/partbypart/icon_primer.png)
Primer
Part:BBa_K4479004:Design
Designed by: Alston Lo, Ruochen Liu, Xinyi Xuan Group: iGEM22_Toronto (2022-10-12)
F3 LAMP Primer for Oak Wilt 2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ranked our F3 primer based on the following criteria: 1. Distance between F2 and F3 primer: 0 - 60 nt 2. Length of F3 primer: 15 - 25 bp 3. GC Content: 40 - 60% 4. Melting temperature for F3 primer: 55 - 63℃ 5. Melting temperature difference between F3 and B3 primers: ≤ 5℃
Source
The part was designed with the Primer Explorer software and then it was ranked against primers provided by the GLAPD software using computer models by our Drylab team. The criteria for ranking were: Primer Distance, Primer Length, GC Content, Melting Temperature, and Melting Temperature Difference. The target sequence it originated from is MG270170.1.